Concept Life Sciences has a strong background in neuroscience and extensive academic links to leading research groups in the field. We help you to understand your MoA and investigate your test compounds across a range of translational models. We share expertise, gathering robust data to drive confident decision making throughout your CNS drug discovery process.
Our experienced neuroscience team offer specialist discovery services to clients looking to develop novel therapies for a range of neurodegenerative conditions. We can tailor and develop neuroprotective screening assays to discover the mechanism of action of your drug candidate, with customized assays targeting neurons themselves and/or the supportive glial cells in isolation or co-culture. We also provide partnering medicinal and computational chemistry, specialist histology, as well as ADME & DMPK services ensuring a comprehensive, tailored CNS program.
Our suite of neuroscience services can be specifically tailored to your project:
- Primary CNS cell-based assays
Ex vivo brain slice assays - RNA and protein-based multiplex histology services
- Brain penetration assessment and receptor occupancy studies
- Neurotoxicity assays
Primary CNS assays
Understanding the effects of your test biologic or small molecule on cells in the CNS is likely a key component of your drug discovery program.
We offer a suite of primary neuronal and glial cell-based assays to help you understand the interaction between your compound or biologic and the nervous system, taking you to clinic faster.
Our highly experienced team can isolate primary CNS cells expressing endogenous targets of interest for use in moderate-throughput phenotypic screening of multiple compounds and MoA studies, helping you achieve accurate, robust results.
Oligodendrocyte precursor cells (OPC)
Understanding how to use to enhance remyelination is key to bringing new treatments to the clinic. We help you understand the role of your drug candidate on oligodendrocyte behavior and myelination.
OPC Assays
We offer screening By assessing the effect of your drug candidate on OPC proliferation, differentiation and apoptosis, we can help you identify compounds with the potential to enhance myelination, bringing you on the best route to the clinic.
We offer screening of primary OPCs (NG2+ cells). A promising therapeutic strategy for Multiple Sclerosis is to promote
differentiation and maturation into oligodendrocytes (MBP+ cells), repairing myelin degeneration. Understanding the effect of your drug candidate on OPCs to enhance remyelination early in discovery is key to bringing new treatments to clinic.
Understanding the remyelination efficiency of your drug candidate in three dimensions offers a translational link between in vitro cell culture and in vivo investigations, a step up in complexity from our screening assay, and your best route to clinic.
Using our organotypic brain slice assays, we can help you understand the functional effects of your compounds. We can determine the degree of axon myelination by oligodendrocytes, during development, we can tailor the assay readouts to your requirements e.g. fluorescent imaging and gene expression analysis by qPCR.
How our OPC assay works
- Mixed glial cultures are prepared and incubated according to protocol
- OPCs are isolated and cultured with test compounds
- Proliferation, apoptosis/toxicity, and differentiation readouts are assessed at relevant timepoints
- Multiplex immunocytochemistry , combined with semi-automated image analysis techniques, allows accurate quantification of compound effects on OPCs
Organotypic brain slice assays
Understanding the functional response to your CNS drug candidate in a more complex in vitro environment can more efficiently progress your drug discovery program to clinic.
We offer brain slice assays as a low-throughput validation model of your test compound in a complex, multicellular approach. Slice cultures contain and maintain all the relevant neuronal and glial cells, structure and organization of the brain in thick sections of cultured ex vivo tissue providing a powerful translational link between in vitro and in vivo CNS studies in important therapeutic areas such as multiple sclerosis, Parkinson’s and Alzheimer’s disease. We also offer a validated slice culture assay to investigate inflammasome activation.
We tailor the assay readouts to suit you. These can include immunohistochemical-based analysis or gene expression studies using qPCR or NGS techniques.
Neurons
Understanding how neurons respond to your drug candidate is key to bringing your therapies to market.
We offer tailored neuronal assays to better understand the MoA of your therapeutic, de-risking your discovery program.
We can screen neurons as isolated neuronal populations e.g. dopaminergic neurons in Parkinson’s Disease or as more complex mixed cell population assays. We can offer custom assay readouts in addition to the standard viability and survival tests as well as neurite outgrowth.
Using our ex vivo slice culture assay, we can study neuronal behavior in the native environment maintaining intact synapses and circuits. With this approach, we aim to generate high-value mechanistic data that can get you to the clinic faster.
Microglia
Microglia are an important cellular target in a range of neurodegenerative diseases such as multiple sclerosis and Alzheimer’s disease
We offer a suite of microglia assays to better understand the MoA of your drug candidate and its potential to enhance the restorative role of activated microglia, de-risking your early discovery program.
We offer monoculture assays using primary cells or cell lines as well as co-culture assays. These assays are ideal for medium-throughput screening and pre-clinical optimization. Working in collaboration with you, we can assess your compound’s ability to dampen the pro-inflammatory response or promote the neuroprotective effector function of activated microglia, Depending on the nature of the compound, we can include a combination of both assessments to investigate the phenotypic switch.
To progress your drug discovery program further, we offer an ex vivo brain slice model as a translational link between in vitro and in vivo studies.
Our scientific team are experts in glial cell biology and work closely with you to customize your program, supporting your best route to clinic.
Astrocytes
Astrocytes are a particular target cell of interest in the nervous system for developing treatments for brain injuries. As the most abundant type of glial cell (non-neuronal cell) in the brain, astrocytes contribute to homeostasis and provide support and protection for neurons. Astrocytes play a fundamental role in the repair and scarring processes of the brain and spinal cord.
Understanding how astrocytes respond to your drug candidate is key to bringing your treatment to market.
We offer a variety of astrocyte assays to help you study the nuances of astrocyte interaction with your drug candidate. We offer customizable monocultures, co-cultures and ex vivo brain slice assays to understand your compound or target.
Whether you are studying neurodegenerative conditions or neuroinflammation, our expert scientists can guide you to answer your questions around astrocyte polarisation and function, speeding up your drug discovery programs.
Neurotoxicity
We are developing a range of neurotoxicity services, including developmental neurotoxicity assays (DNT), using our pluripotent cell and primary CNS cell-based assay platforms that can support Pharma and non-Pharma clients in their regulatory and safety requirements.