Genetic Modification

Advanced techniques to modify genes

Genetic modification is a powerful tool for altering gene expression. Our experienced team of molecular biologists offer a range of techniques for delivering gene alterations to your cell of interest. Whether the goal is to deliver therapeutic modification or interfere with RNA transcription, we will optimize conditions for delivery, determine transfection efficiency and identify downstream effects. In partnership with our Cell Based Assays, Immunology and Neuroscience teams we will provide bespoke assays to confirm functional effects in vitro and in vivo.

shRNA (short hairpin RNA)

shRNA are synthetic RNA molecules designed to induce RNA interference (RNAi). shRNA can be expressed within cells, forming a hairpin structure that is processed into small interfering RNAs leading to their degradation and subsequent gene silencing. Commonly used to create stable cell lines with desired genetic modifications for long-term studies

siRNA (small interfering RNA)

siRNA are a class of double-stranded RNA molecules designed to specifically target and degrade complementary mRNA sequences. This mechanism allows for precise and transient gene silencing, making siRNA a valuable tool for functional genomics, drug target validation, and therapeutic development

Viral transfection

Lentiviral and adeno-associated viral (AAV) are industry standard techniques for efficiently delivering genetic material to a wide range of cell types without inducing an adverse immune reaction. We can generate appropriate viral vectors, identify MOI and efficiency of transfection, then confirm the effect on target protein expression.

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