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Target and Pathway Engagement Assays
Confirm on-target efficacy and accelerate clinical success
Do you have confidence in your candidates' cellular activity?
Achieving clinical success starts with precision and confidence in your candidates cellular activity. At Concept Life Sciences, our cell-based target and pathway engagement assays enable drug discovery teams to confirm on-target activity and make data-driven decisions with confidence.
Cell-based target and pathway engagement assays
Designed to integrate seamlessly into your design-make-test-analyze (DMTA) cycle, these assays demonstrate that your compound engages its molecular target in live cells, providing essential insights for hit validation, lead optimization and structure-activity relationship (SAR) studies. They are:
- Customizable to your therapeutic indications and targets
- Backed by expert consultation and advanced data interpretation
On-target efficacy solutions
We specialize in high-quality, customizable cell-based assays for validating hit-to-lead and compounds across multiple pathways. Our services support every stage of the drug discovery pipeline, particularly during:
- Hit-to-lead progression
- Lead optimization
- Target validation for structure-activity studies
Assay capabilities include:
- Cyclic AMP (cAMP) / cyclic GMP (cGMP): Glo™ assays, NanoBRET, AlphaScreen
- Calcium flux assays: Calcium flux measurement, FLIPR
- Inositol triphosphate (IP3): IP-one assay
- Protein quantification: HTRF, AlphaLISA, western blotting, flow cytometry, Jess
- Caspase-1 activation: Caspase-Glo® 1
- β-Arrestin activation: HTRF
For an example of an application of target and pathway engagement assay read the NLRP3 inflammasome case study
FAQs:
Q: What are target and pathway engagement assays?
A: Cell-based assays that confirm a compound engages its intended molecular target and pathway in live cells, providing critical efficacy data.
Q: Why are target and pathway engagement assays important?
A: They validate mechanism-of-action, support structure-activity relationship (SAR) studies, and reduce the risk of advancing inactive or off-target compounds.
Q: Can assays be customized for specific therapeutic areas?
A: Yes. Each assay can be tailored to your targets, disease context, and project requirements.
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