B Cell Activation Assays

B cell activation and function are central to adaptive immunity, playing key roles in antibody production, antigen presentation, and immune memory.

Modulating B cell activity is important across multiple applications, including enhancing responses for vaccine development or oncology, and reducing activity in autoimmune diseases where pathogenic antibodies or aberrant activation drive disease. This assay uses human primary immune cells to evaluate how compounds influence B cell activation and differentiation.

• Modality agnostic: Suitable for both biologics and small molecules.
• Flexible formats (PBMC or isolated CD19+ B cells) to capture system-level or B cell-intrinsic effects.
• Multiple stimulation strategies (CD40L, anti-Ig, CpG) to tailor assay to specific biology.
• Diverse functional readouts covering proliferation, activation, and antibody production.

• Flow cytometry quantifies B cell proliferation and activation marker expression (e.g. CD27 and CD38).
• Antibody production (e.g. total and IgG subclasses) measured using ELISA or multiplex platforms.
• Data demonstrate differential responses depending on stimulation conditions and compound treatment.
• Results provide insight into both functional activation and downstream antibody responses.

This assay enables detailed interrogation of B cell responses across activation and differentiation stages.

By integrating multiple stimulation strategies with functional and phenotypic readouts, it supports a deeper understanding of how compounds modulate humoral immunity, helping guide development in areas such as autoimmunity, vaccination, and oncology.