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Primary Cells

Primary cell cultures, derived directly from organ tissue, closely mimic the physiological conditions of in vivo cells, offering biologically relevant data. These cultures serve as a superior model for preclinical and investigative research, capturing intercellular and intracellular communication intricacies. Unlike immortalised cell lines, which are prone to mutations and chromosomal abnormalities, primary cells provide a more accurate representation of tissue cell types.

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A more in-depth view

Concept Life Sciences is at the forefront of scientific and technical expertise, particularly in working with primary human and rodent cells, with a specialized focus on immune and central nervous system cells.

  • Comprehensive Protocols: Benefit from our developed and robust protocols, covering isolation, culture, differentiation, and activation of primary human and rodent cells.
  • Versatile Applications: Our models excel in both simple monoculture and complex co-culture assay systems, effectively recapitulating key pathophysiological mechanisms. These models find diverse applications across various disease indications, including immunology, immuno-oncology, auto-immunity, inflammation, and neuroinflammation.
  • Advanced Models: Cultivated with suitable primary cell culture media and supplements, our models represent advanced and accurate reflections of normal cell phenotypes and early disease progression.

Explore our collection of monoculture and co-culture assays by cell type:

T cell: CD3+, CD4+, CD8+ subsets, T cell activation assay, tailored read-outs: viability, proliferation, marker expression, cytokine production; T cell-mediated killing of cancer cells, T cell differentiation into Th1, Th17 and iTreg T cell subtypes; T cell suppression assay, migration assay.

Regulatory T cells: inducible Treg (iTreg) differentiation, nTreg isolation, T cell suppression assay.

NK cells: NK cell phenotyping and activation assay; natural cytotoxicity, antibody-mediated cytotoxicity assay.

B cell: B cell activation assay.

Macrophage: macrophage differentiation assay, reprogramming assay, M1- and M2-like macrophage differentiation, phagocytosis, T cell suppression.

Dendritic cells: dendritic cell activation and maturation, antigen presentation to T cells, T cell activation assay, immunogenic cell death.

Neurons: Neurotoxicity and Neuroprotection assays.

Astrocytes : Activation Assay

Microglia : Activation Assay, phagocytosis assay, migration.

Oligodendrocyte Precursor Cells : Proliferation and maturation assays

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