We have extensive experience of in vitro screening and deliver customised, instructive and efficient screening programs to progress fully characterised hits through drug discovery programs. We offer medium-throughput screening approaches using biophysical, biochemical, phenotypic and cell-based assays. Our technologies allow cost-effective identification of hit compounds from any type of compound library, including our proprietary fragment library.Connected servicesMore informationSpeak to an expert
Biochemical screening is a crucial initial step in drug discovery, involving the evaluation of potential drug candidates by testing their interactions with isolated biological molecules. In this process, we use purified enzymes, receptors, or other biomolecules to assess how a compound influences specific biochemical reactions. We offer ultras high-throughput (UHT) screening methods through our trusted partner to analyse large compound libraries rapidly. This approach will allow you to identify molecules that exhibit promising interactions with the target biomolecule. The successful hits from your biochemical screen can then progress to further stages of drug development, serving as a foundation for understanding the molecular mechanisms underlying drug-target interactions.
Biophysical screening complements biochemical methods by providing insights into the physical properties of drug-target interactions. We offer techniques such as surface plasmon resonance (SPR), grating-coupled interferometry (GCI), isothermal titration chromatography (ITC), differential scanning fluorimetry (DSF), nuclear magnetic resonance (NMR), and X-ray crystallography to study the structural aspects of molecular binding. These biophysical methods offer a more detailed understanding of how a drug candidate interacts with its target on a molecular level, providing information on binding affinities, kinetics, and thermodynamics. This structural information is crucial for optimizing your lead compounds and guiding medicinal chemistry efforts to enhance the potency and selectivity of your potential drug.
Cellular screening involves evaluating the effects of potential drug candidates within a cellular context, providing a more physiologically relevant assessment of drug activity. We use cell-based assays to study how compounds influence cell viability, proliferation, or specific cellular functions. This step is essential for understanding how a drug may behave in a complex biological environment, considering factors such as cell permeability, metabolism, and potential off-target effects. Cellular screening helps bridge the gap between the early stages of drug discovery and the later stages involving animal models and clinical trials, ensuring that promising compounds identified through our biochemical and biophysical screening translates well into functional outcomes within living systems.