Start your own journey to clinic

Please provide us with a few details so that we can match you with the right expert in your field.


Thank you, your request has been received.

We're busy connecting you with an expert, who will be in touch as soon as possible.

Oops! Something went wrong while submitting the form. Please try again or email us at


Developed and validated LC-MS/MS assays for T4 and Thyroxin to study in vitro UGT induction in rat and human hepatocytes."

Uridine 5'-diphospho-glucuronosyltransferases (UGTs) are phase II enzymes foundin the liver and are responsible for glucuronidation of metabolites to facilitatesystemic elimination.• Circulating concentrations of thyroid hormone (TH) are decreased throughinduction of thyroxine (T4) glucuronidation by many xenobiotics which furtherleads to increased secretion of thyroid-stimulating hormone, causing increasedthyroid follicular cell proliferation, hypertrophy and ultimately follicular celladenomas/carcinomas (Figure-1).• This disruption is due to an increased expression and activity of UGTs particularlyinvolved in the glucuronidation of T4.• An in vitro comparative UGT assay showing that UGT is induced in rodents but notin humans could establish that any subsequent key events and adverse outcomesassociated with this mechanism of action will not occur in humans and thus arenot considered relevant to humans due to differences in TH physiology.• An in vitro method for primary rat and human hepatocytes was developed forassessment of induction of UGT gene expression and enzyme activity. LC-MS/MSassays were developed and validated for the simultaneous determination ofThyroxine (T4) and Thyroxine-o-β-D-Glucuronide (T4-Gluc) concentrations in bothrat and human hepatocyte media supernatant samples, to assess UGT enzymeactivity.

Alternatively, search our entire resource library below

Show filters
View all
Category one
Category two
Category three
Category four
Resource type
Related service line
Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.
Showing 0 items
No results found.
There are no results with this criteria. Try changing your search.