Protein Expression and Purification
Protein expression involves the synthesis of a target protein in a host organism, commonly using recombinant DNA technology. We offer expression in bacteria, yeast, insect cells, and mammalian cells. depending on the complexity of the protein, post-translational modifications required, and the intended application.
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Protein Expression
After introducing the recombinant DNA, we can optimise the expression process within the host cells using techniques such as gene cloning, transformation, and induction, allowing us to generate large quantities of recombinant proteins for various applications, including structural studies, drug development, and therapeutic protein production.
Protein Production & Purification
Following protein expression, the next critical step is production, which entails the cultivation of the host cells at a large scale to generate sufficient quantities of the recombinant protein. We optimise growth conditions and media composition to maximize protein yield while maintaining product quality. Subsequently, the harvested cell culture undergoes purification, a crucial process to isolate the target protein from other cellular components. We utilise various chromatographic methods, such as affinity chromatography, ion-exchange chromatography, and size-exclusion chromatography, to purify the protein ensuring the production of highly pure and bioactive proteins.