Histology Services

Latest laboratory equipment and cutting-edge technologies

Concept Life Sciences Histology utilizes the latest laboratory equipment and cutting-edge technologies to provide end-to-end histology services. We follow the guidelines set out by Good Clinical Practice (GCP) and therefore can support projects throughout the drug discovery and development process, from early-stage R&D through to clinical trials.

Concept Life Sciences Histology is set up to Source, Process, Stain, Image, and Analyze tissue.

Staining approaches provided by Concept Life Sciences Histology

Our use of the latest laboratory equipment, including the Leica BOND RX Research Stainer, and PhenoImagerTM HT and Zeiss Axioscan imaging systems, enables us to fully automate many of our staining approaches allowing us to deliver a robust, reliable, and high-throughput service.

Tissue Stains

Including but not limited to H&E, Alcian blue, PAS, and Picrosirius red.

Single and multiplex chromogenic immunohistochemistry (IHC)

Up to 3 markers pus nuclear stain (Haematoxylin)
e.g., DAB, Fast Red red, Green, green, and Blue

Single and multiplex immunofluorescence (IF)

Up to 8 markers plus nuclear stain (DAPI).

RNAscope

mRNA detection; up to 4-plex

Mixed multiplex detection

IHC (protein) + RNAscope (mRNA)

Staining dye

[CLS Histology staining dye HE.jpg] CLS Histology staining dye HE.jpg

H&E

(human NSCLC)

Staining dye

[CLS Histology staining dye alcian blue.jpg] CLS Histology staining dye alcian blue.jpg

Alcian blue

(human colon)

Chromogenic IHC

[CLS Histology chromogenic IHC CD3 haematocylin.jpg] CLS Histology chromogenic IHC CD3 haematocylin.jpg

CD3, Haematoxylin

(human NSCLC)

2-plex IHC

[CLS Histology 2-plex ICH mouse.jpg] CLS Histology 2-plex ICH mouse.jpg

CD8, CD31, Haematoxylin

(mouse spleen)

[CLS Histology IF human.jpg] CLS Histology IF human.jpg

CD8, DAPI
(human NSCLC)

2-plex IF

[CLS Histology 2-plex IF mouse kidney.jpg] CLS Histology 2-plex IF mouse kidney.jpg

PanCK, C4d, DAPI

(mouse kidney)

3-plex IF

[CLS Histology 3-plex IF human tonsil.jpg] CLS Histology 3-plex IF human tonsil.jpg

CD68, CD8, PanCK, DAPI

(human tonsil)

4-plex IF

[CLS Histology 4-plex IF human tonsil.jpg] CLS Histology 4-plex IF human tonsil.jpg

PD-L1, PD-1, CD4, CD8, DAPI

(human tonsil)

RNAscope

[CLS Histology RNAscope HeLa cellsl.jpg] CLS Histology RNAscope HeLa cellsl.jpg

POLR2A, DAPI

(HeLa cells)

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8-plex (9-colour) IF

Concept Life Sciences Histology offers simultaneous imaging of up to 8 markers (proteins) + DAPI (nuclear stain) on a single tissue section. This can provide morphological, pathological, and spatial information of the juxtaposition of different cell types, which can be quantified by image analysis.

Image left: CD3, CD8, CD4, FoxP3, CD68, Ki67, PanCK, DAPI with OpalTM Multiplex Detection Reagents imaged using the PhenoImager HT from Akoya Biosciences (human CRC)

Tissue biomarkers

Specialized IHC staining approaches utilized during preclinical and clinical drug discovery research provide valuable insights into the location and positioning of biomarkers (cell types) within tissue.

Our knowledge, expertise and pragmatic approach to antibody selection and validation can facilitate demonstration of novel and challenging biomarkers in situ, providing high-value data.

Image right: Human tonsil – T cells (CD3), Cytotoxic T cells (CD8), Regulatory T cells (FoxP3), Myeloid cells (CD68), Epithelial cells (PanCK), Proliferating cells (Ki67), Nuclei (DAPI)

Related services

Areas of expertise

Our areas of expertise include:

Immunology
Immuno-oncology
Fibrosis
Neuroscience