Latest laboratory equipment and cutting-edge technologies
Concept Life Sciences Histology utilizes the latest laboratory equipment and cutting-edge technologies to provide end-to-end histology services. We follow the guidelines set out by GCP and therefore can support projects throughout the drug discovery and development process, from early-stage R&D through to clinical trials.
Concept Life Sciences Histology is set up to Source, Process, Stain, Image, and Analyze tissue.
Staining approaches provided by Concept Life Sciences Histology
Our use of the latest laboratory equipment, including the Leica BOND RX Research Stainer and Vectra PolarisTM imaging system enables us to fully automate many of our staining approaches enabling us to deliver a robust, reliable, and high-throughput service.
Staining dyes
e.g., H&E, Alcian blue, Picrosirius red
Single and multiplex chromogenic immunohistochemistry (IHC)
e.g., DAB, red, green
Single and multiplex immunofluorescence (IF)
up to 8-plex (9-colour incl. DAPI)
RNAscope
mRNA detection; up to 4-plex
Mixed multiplex detection
IHC/IF (protein) + RNAscope (mRNA)
| Staining dye
H&E (human NSCLC) |
Staining dye
Alcian blue (human colon) |
Chromogenic IHC
CD3, Haematoxylin (human NSCLC) |
| 2-plex IHC
CD8, CD31, Haematoxylin (mouse spleen) |
IF
CD8, DAPI |
2-plex IF
PanCK, C4d, DAPI (mouse kidney) |
| 3-plex IF
CD68, CD8, PanCK, DAPI (human tonsil) |
4-plex IF
PD-L1, PD-1, CD4, CD8, DAPI (human tonsil) |
RNAscope
POLR2A, DAPI (HeLa cells) |
![[CLS Histology staining dye HE.jpg] CLS Histology staining dye HE.jpg](https://www.conceptlifesciences.com/site/uploads/2023/02/CLS-Histology-staining-dye-HE_Original-file-1.webp)
![[CLS Histology staining dye alcian blue.jpg] CLS Histology staining dye alcian blue.jpg](https://www.conceptlifesciences.com/site/uploads/2023/02/CLS-Histology-staining-dye-alcian-blue_Original-file.webp)
![[CLS Histology chromogenic IHC CD3 haematocylin.jpg] CLS Histology chromogenic IHC CD3 haematocylin.jpg](https://www.conceptlifesciences.com/site/uploads/2023/02/CLS-Histology-chromogenic-IHC-CD3-haematocylin_Original-file.webp)
![[CLS Histology 2-plex ICH mouse.jpg] CLS Histology 2-plex ICH mouse.jpg](https://www.conceptlifesciences.com/site/uploads/2023/02/CLS-Histology-2-plex-ICH-mouse_Original-file.webp)
![[CLS Histology IF human.jpg] CLS Histology IF human.jpg](https://www.conceptlifesciences.com/site/uploads/2023/02/CLS-Histology-IF-human_Original-file.webp)
![[CLS Histology 2-plex IF mouse kidney.jpg] CLS Histology 2-plex IF mouse kidney.jpg](https://www.conceptlifesciences.com/site/uploads/2023/02/CLS-Histology-2-plex-IF-mouse-kidney_Original-file.webp)
![[CLS Histology 3-plex IF human tonsil.jpg] CLS Histology 3-plex IF human tonsil.jpg](https://www.conceptlifesciences.com/site/uploads/2023/02/CLS-Histology-3-plex-IF-human-tonsil_Original-file.webp)
![[CLS Histology 4-plex IF human tonsil.jpg] CLS Histology 4-plex IF human tonsil.jpg](https://www.conceptlifesciences.com/site/uploads/2023/02/CLS-Histology-4-plex-IF-human-tonsil_Original-file.webp)
![[CLS Histology RNAscope HeLa cellsl.jpg] CLS Histology RNAscope HeLa cellsl.jpg](https://www.conceptlifesciences.com/site/uploads/2023/02/CLS-Histology-RNAscope-HeLa-cellsl_Original-file.webp)
8-plex (9-colour) IF
Concept Life Sciences Histology offers simultaneous imaging of up to 8 markers (proteins) + DAPI (nuclear stain) on a single tissue section. This can provide morphological, pathological, and spatial information of the juxtaposition of different cell types, which can be quantified by image analysis.
Image left: CD3, CD8, CD4, FoxP3, CD68, Ki67, PanCK, DAPI with OpalTM Multiplex Detection Reagents imaged using the Vectra PolarisTM from PerkinElmer (human CRC)
Tissue biomarkers
Specialized IHC staining approaches utilized during preclinical and clinical drug discovery research provide valuable insights into the location and positioning of biomarkers (cell types) within tissue.
Our knowledge, expertise and pragmatic approach to antibody selection and validation can facilitate demonstration of novel and challenging biomarkers in situ, providing high-value data.
Image right: Human tonsil – T cells (CD3), Cytotoxic T cells (CD8), Regulatory T cells (FoxP3), Myeloid cells (CD68), Epithelial cells (PanCK), Proliferating cells (Ki67), Nuclei (DAPI)
Areas of expertise
Our areas of expertise include:
- Immunology
- Immuno-oncology
- Fibrosis
- Neuroscience