The thyroperoxidase (TPO) assay uses thyroid gland microsomal protein from the relevant species. TPO activity is determined by monitoring the oxidation of guaiacol using H2O2 as a hydrogen donor.
Thyroperoxidase is found in thyroid follicular cells. TPO catalyzes the oxidation and incorporation of iodide into thyroglobulin with subsequent production of thyroid hormones. Differences in species sensitivity to TPO inhibitors are known and thus we perform a cross-species set of assays to support the interpretation of human relevance.
Deliverable: Study report detailing protocol, data and IC50 of test compound.
