Spatial interrogation of biomarkers within the tumour microenvironment provides valuable insight into tumour progression and drug response. Development of reliable
multiplex IHC / IF staining assays is however complex, time-consuming, and relies upon antibody suitability. To address this, we aimed to adopt two novel assays;
PhenoCodeTM Signature Panels and RNAscope + multiplex IHC, which offer an alternative approach to biomarker discovery and development. PhenoCode
TM Signature
Panels greatly reduce panel optimisation times by utilising barcode-based antibody chemistry to apply a single pre-optimised cocktail of six antibodies in one step.
RNAscope + multiplex IHC enables the detection of multiple RNA and protein biomarkers on the same tissue section, providing a solution when a suitable antibody cannot
be sourced for a particular biomarker. Here we test the robustness of a PhenoCode
TM panel and demonstrate the staining of two RNAscope / protein panels as proof of
principle.
Novel Assays to Enhance Spatial Biomarker Discovery and Development
