The Steroidogenesis H295R assay is an in vitro test system used to investigate compound effects on steroid hormone biosynthesis, specifically 17β-estradiol (E2) and testosterone (T).
The human H295R adreno-carcinoma cell line expresses genes that encode all the key enzymes for steroidogenesis.
This assay is performed according to the OECD test guideline No456R with LC-MS/MS quantitation. Cell viability is checked to enable accurate interpretation of results.
Deliverable: Study report detailing protocol and data; LOEC/Max fold change of test compound.
