Chromatographic Hydrophobicity Index (CHI) is a physicochemical method that utilizes the fundamental chemistry behind gradient reverse phase liquid chromatography (RP-LC) to determine hydrophobicity. In gradient RP-LC the retention time (tR) of a compound is related to the ratio of Organic Mobile Phase (OMP) and aqueous mobile phase (AMP) required for a compound to elute. A compound’s relationship with Polarity makes tR a good indicator for the hydrophobicity of a compound, i.e. Highly retained compounds requiring high organic mobile phase content for elution suggests high hydrophobicity.
Deliverable: CHI LogD7.4 value.
