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Immune and
cell-based assays

Concept Life Sciences develop robust in vitro immune and cell-based assays for proof-of-concept, screening and mechanism of action studies using project relevant primary cells or immortalised cell line models.

Off-the-shelf and novel immune assays

Concept’s Immunology team have developed a wide range of immune cell-based assays, which are available off-the-shelf or fully tailored for your program. We support our clients to select the best assay design for the project, providing support through in vitro and in vivo proof-of-concept and mode-of-action studies in immunology, oncology and neurodegenerative diseases as well as biomarker discovery and target expression for other immune-related diseases.

Our state-of the-art laboratories incorporate cutting-edge equipment such as the IncuCyte® Live Cell Analysis System for visualisation and quantification of cell behaviour over time and the Leica Microsystems THUNDER Imager 3D Live Cell & 3D Cell Culture & 3D Assay platform to deliver high quality and robust data in our service offerings.

We advise on the most suitable cell types and cell lines, mono- or co-culture assay systems, and provide tailored readouts to build a data package that is right for you.

These include cytokine expression by ELISA, multiplex, or MSD, proliferation and cell surface markers by multi-colour flow cytometry, and gene expression by qPCR or NGS. Our 96- and 384-well plate formats allow for moderate to high-throughput screening of multiple compounds and dose responses, achieving fast, precise results.

At Concept, we have extensive experience at developing completely novel assays as well as full experimental programs for small molecules, biologics, bispecifics and CAR-Ts.

Immune and cell-based assay capabilities

Our assays include:

  • MLRs
  • PBMC and T cell stimulation assays
  • Exhausted T cell assay
  • Killing assays
  • Suppression assays
  • Myeloid cell assays
  • Phagocytosis assays
  • Cytotoxicity assays
  • Customised cell-based assays

    Concept has highly experienced cell biologists able to deliver a wide range of customisable cell-based assays for compound study and safety profiling as part of a drug discovery program. Our cellular assays are relevant across the whole drug discovery process from target discovery through to lead optimisation and candidate selection.

    Working consultatively with clients, our scientists efficiently evaluate project requirements and provide support no matter where in the drug discovery process a project resides.

    We use an array of latest technologies and automation platforms, including digital dispensing and liquid handling, allowing us to reduce sample volume/reagents and ensure fast turnaround times.

    All assays are designed to be robust and reproducible, generating high quality, reliable data. Our dedicated scientists are available to provide support interpreting results and can offer on-going guidance throughout the project lifetime.

    We can design cellular assays for a wide variety of purposes including:

    • Proof-of-concept
    • Compound profiling and screening
    • Target discovery/validation
    • Mechanism of action studies
    • Elucidation of the disease biology

    We offer a wide range of in vitro primary cell assays that focus on isolated primary cells and/or a co-culture system. We have experience and access to the following cell types:

    • All immune cells
    • Primary CNS cells
    • Hepatocytes
    • Keratinocytes
    • Ex vivo tumour samples
    • Cellular target engagement and phenotypic assays with readouts including
      • FI
      • Absorbance
      • Luminescence
      • FP
      • HTRF
      • FRET/BRET
    • Fast kinetic assays e.g. ion channel, GPCR
    • Mechanism of action studies
    • Cell proliferation and viability assays
    • Western blotting and ELISAs to measure changes in protein expression and post-translational modification
    • Biomarker analysis

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Biochemical assays

Concept Life Sciences’ biology department have extensive experience in developing and validating biochemical assays, performing both client assay-transfer programs or off-the-shelf assays from commercial vendors. We deliver high-quality, consistent, cost-effective assay data to our clients with an emphasis on fast and efficient turnaround times.

Biophysical assays

Concept Life Sciences' biology department is renowned for its biophysical assay development, screening and data interpretation. Extensive biophysical assay expertise gained within leading pharma allows us to employ the most appropriate biophysical techniques for any given target.

Gene expression

Concept Life Sciences combines state-of-the-art technologies with a customised service offering to meet gene expression requirements. Offered as a stand-alone service or as a flexible, tailored readout to one of our immune cell-based assays, we help harness the power of Next Generation Sequencing (NGS) -based technologies including NanoString, single-cell and bulk RNA-sequencing, as well as qPCR for target gene expression analysis.