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Biophysical assays

Concept Life Sciences' biology department is renowned for its biophysical assay development, screening and data interpretation. Extensive biophysical assay expertise gained within leading pharma allows us to employ the most appropriate biophysical techniques for any given target.

Concept Life Sciences offers a wide range of biophysical assays that provide important information on target engagement throughout the drug discovery process.

We use our biophysical capabilities from hit finding to lead optimisation, including extensive use in our fragment-based lead discovery programs. Our biophysical techniques can also provide a full mechanistic, structural, kinetic, and thermodynamic profile of compounds at all stages of the project.

 

Biophysical screening, assay development and characterisation

Our teams use an array of biophysical techniques for screening purposes, and to increase the understanding of compound-target interactions. These techniques can be used as primary screens, e.g. SPR or DSF in fragment screening, or as orthogonal confirmatory assays in biochemical and cellular screening cascades.

Techniques range from simple thermal shift assays to more advanced techniques, such as microscale thermophoresis (MST) using NanoTemper instrumentation, isothermal titration calorimetry (ITC) using MicroCal PEAQ-ITCs and surface plasmon resonance (SPR) using Biacore.

In combination these techniques can provide vital affinity, stoichiometric, kinetic and thermodynamic data on ligands binding to their target.

 

All of the biophysical techniques mentioned are equally applicable to small molecules, peptides and biologics, including antibodies.

Capabilities

  • DSF (Differential Scanning Fluorimetry) using RT-PCR instruments
    • Used for: Library screening (including fragment libraries), orthogonal hit verification, hit validation, SAR and affinity profiling (Kd)
  • SPR (Surface Plasmon Resonance) using either a Biacore T200 or a Biacore 8k
    • Used for: Library screening (including fragment libraries), orthogonal hit verification, hit validation, SAR, affinity profiling (Kd) and kinetic profiling (kon, koff, residence time)
  • ITC (Isothermal Titration Calorimetry) using a MicroCal PEAQ-ITC
    • Used for: Protein QC, validation of other biophysical techniques, orthogonal hit verification, hit validation, SAR, affinity profiling (Kd) and thermodynamic profiling (ΔG, ΔH, ΔS)
  • MST (MicroScale Thermophoresis) using a NanoTemper NT115pico
    • Used for: Protein QC, validation of other biophysical techniques, library screening (including fragment libraries), orthogonal hit verification, hit validation, SAR and affinity profiling (Kd)

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Biochemical assays

Concept’s knowledge of assay development gained over many years within the pharmaceutical industry allows us to develop, optimise and validate bespoke high-quality biochemical assays during any part of the drug discovery process.

Custom assay development

Concept has extensive experience in the use of a wide range of in vitro assays (biochemical, biophysical and cellular including immune-cell based) for proof-of-concept, screening, characterisation and validation of compounds and biologics.

Immune and cell-based assays

Concept Life Sciences develop robust in vitro immune and cell-based assays for proof-of-concept, screening and mechanism of action studies using project relevant primary cells or immortalised cell line models.